Microfluidic Hydrodynamic Cell Separation: A Review
نویسندگان
چکیده
Microfluidic continuous cell separation based on hydrodynamic interaction in a microfluidic channel has attracted attention because of its robustness, high throughput and cell viability. This paper systematically gives an overview on recent advances in hydrodynamic particle and cell separation in microfluidic devices. It presents the basic ideas and fluid mechanics for the hydrodynamic interaction of a particle in a microfluidic system. Secondly, different kinds of devices are introduced with detailed descriptions of their mechanisms, designs and performances. Finally, the review addresses some practical issues of microfluidic sorting devices for use in biological or medical studies.
منابع مشابه
Microfluidic continuous particle/cell separation via electroosmotic-flow-tuned hydrodynamic spreading
Among the microfluidic separation methods, hydrodynamic spreading is a simple and high-throughput continuous separation technique based on the difference in size. However, it is difficult to adjust tiny pressure differences accurately in microfluidic devices. In this study, a combination of electroosmotic flow (EOF) and hydrodynamic flow spreading was employed to tune the size separation of par...
متن کاملRecent advances in microfluidic cell separations.
The isolation and sorting of cells has become an increasingly important step in chemical and biological analyses. As a unit operation in more complex analyses, isolating a phenotypically pure cell population from a heterogeneous sample presents unique challenges. Microfluidic systems are ideal platforms for performing cell separations, enabling integration with other techniques and enhancing tr...
متن کاملHandling and analysis of cells and bioparticles on centrifugal microfluidic platforms.
Microfluidic systems for cell separation and analysis have attracted increasing research activity over the past decades. In particular, the prospect of integrating all steps from sample preparation to assay readout in a single microfluidic cartridge, which is inserted into a compact, portable and potentially low-cost instrument, bears great promise to leverage next-generation diagnostic product...
متن کاملMicrofluidic Device for Continuous Particle Separation Using Hydrodynamic Filtration
A size-dependent particle separation (classification) is one of the most important procedures in biochemical, environmental, or medical analyses and their applications. However, the smaller particle size or the smaller size difference makes the particle classification difficult. The Lab-on-a-Chip concept have encouraged the miniaturization of various separation or selection methods for small pa...
متن کاملLabel-free cell separation and sorting in microfluidic systems
Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods. Recently, there has been interest in methods which avoid the use of biochemical labels; numerous intrinsic biomarkers have been explored to identify cells including size, electrical polarizability, and hydrodynamic properties. This review highlights microfluidic techniques us...
متن کامل